|
KMID : 0363320150360030217
|
|
Journal of Korean Oriental Internal Medicine
2015 Volume.36 No. 3 p.217 ~ p.227
|
|
|
Characteristic Effects of Dangnyo-hwan for Diabetes Control Studied Using LC-MS/MS and ICP
|
|
In Jeong-Do
Im Dai-Sig
Moon Seung-Ho
Kim Won-Ill
|
|
|
Abstract
|
|
|
Objectives: The primary aim of this study was to identify bioactive compounds in Dangnyo-hwan, a Korean herbal medicine, through instrumental analysis using LC-MS/MS and ICP, and investigate its potential use in diabetes treatment.
Methods: The extract of Dangnyo-hwan has 12 medicinal herbs; these were compared with 18 marker substances selected from literature survey.
Results: LC-MS/MS analysis could detect 9 of the 18 bioactive compounds: citruline, catalpol, berberine, ginsenoside Rb1, ginsenoside Rg1, oleanolic acid, β-sitosterol, mangiferin, and schizandrin. While harmful heavy metals such as As, Pb, Cd, Hg, Ni, and Cu were not present in high concentrations, Zn concentration was 4.2 mg in 100 g Dangnyo-hwan.
Conclusions: Instrumental analysis such as LC-MS/MS and ICP was successfully used to identify bioactive compounds in Dangnyo-hwan. Detection of 9 bioactive substances and Zn from the herb medicine is a valuable finding, and suggests that Dangnyo-hwan is a candidate medicine for diabetes. Further investigations like in vitro assay, percent GPR 119 activity, and percent human DGAT-1 inhibition are underway.
|
|
|
KEYWORD
|
|
|
Dangnyo-hwan, diabetes, LC-MS/MS, ICP, marker substances
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|